The 13th Annual Patton Lecture

Professor Bryony Bonning

Iowa State University

Plant Virus-Aphid Vector Molecular Interactions and Associated Technologies

Aphids transmit more than 275 plant viruses that result in considerable economic loss within the agricultural sector. Viruses in the Luteoviridae are obligately transmitted by aphids in a persistent manner that requires virion accumulation in the aphid hemocoel. To enter the hemocoel, the virion must bind and traverse the aphid gut epithelium. The molecular mechanisms involved in this process are poorly understood. By screening a phage display library, we identified a peptide that binds to the gut epithelium of the pea aphid Acyrthosiphon pisum (Harris) and impedes the update of Pea enation mosaic virus (PEMV) from the pea aphid gut into the hemocoel. In this talk, identification of the gut receptor for PEMV and the development of two novel aphid management technologies based on knowledge of pea aphid – PEMV molecular interactions will be described. These technologies provide enhanced delivery of both gut active and neurotoxic peptides.

2014 Patton Symposium:  Angela Douglas, lecturer Bryony Bonning, Nicolas Buchon and Jeff Scott