My laboratory uses the genetic model Drosophila melanogaster to study the host response to indigenous and pathogenic microbes using genomics, functional genetics and systems-level approaches. We focus on the integration of immune , growth and repair mechanisms. In addition, we study intestinal physiology, and focus on how intestinal stem cells integrate signals from their environment, including nutrients, stress, or metabolites from the microbiota
Our lab is interested in understanding Host-Microbe relationships and the genetic network that governs the host response to microbes. Our goal is to integrate the immune response per se with mechanisms employed by the host to repair the infectious damage in an integrative model. The main focus of our work is to characterize the host mechanisms that control intestinal homeostasis in response to infectious and resident microbes. In addition, we want to understand how microbes can alter intestinal homeostasis and give rise to pathologies of gut origin such as intestinal immune disorders or cancers. Finally we aim to characterize the spatial complexity of the gastro-intestinal system, and we study how gut regions are established and maintained and how regional variations in intestinal physiology are relevant to health and disease. We emphasize coupling between genomics, functional genetics, cell biology and systems level approaches (computation and theory) and use the fruit fly as our main model system.
I teach two primary courses. One is a course of insect physiology shared with Angela Douglas. The second is a course of comparative immunology: innate immunity in action.
- Buchon, N., & Osman, D. (2015). All for one and one for all: Regionalization of the Drosophila intestine. Insect Biochemistry and Molecular Biology.
- Sansone, C. L., Cohen, J., Yasunaga, A., Xu, J., Osborn, G., Subramanian, H., Gold, B., Buchon, N., & Cherry, S. (2015). Microbiota-Dependent Priming of Antiviral Intestinal Immunity in Drosophila. Cell host & microbe.
- Dutta, D., Buchon, N., Xiang, J., & Edgar, B. A. (2015). Regional Cell Specific RNA Expression Profiling of FACS Isolated Drosophila Intestinal Cell Populations. Current Protocoles in Stem Cell Biology.
- Dutta, D., Dobson, A. J., Houtz, P. L., Gläßer, C., Revah, J., Korzelius, J., Patel, P. H., Edgar, B. A., & Buchon, N. (2015). Regional Cell Specific RNA Expression Profiling of FACS Isolated Drosophila Intestinal Cell Populations. Cell Reports.
- Xiao, H., wang, H., Silva , E. A., Thompson, J., Guillou, A., Yates Jr, J. R., Buchon, N., & Franc, N. C. (2015). The Pallbearer E3 ligase promotes actin remodeling via RAC in efferocytosis by degrading the ribosomal protein S6. Developmental Cell.
- Buchon, N., Silverman, N., & Cherry, S. (2014). Immunity in Drosophila melanogaster--from microbial recognition to whole-organism physiology. Nature Reviews Immuno.
- houtz, p. l., & Buchon, N. (2014). Methods to assess intestinal stem cell activity in response to microbes in Drosophila melanogaster. Methods in molecular biology (Clifton, N.J.).
- Broderick, N. A., Buchon, N., & Lemaitre, B. (2014). Microbiota-induced changes in drosophila melanogaster host gene expression and gut morphology. mBio.
- osman, D., & Buchon, N. (2014). Unexpected complexity of the Drosophila digestive tract: towards a model for functional regionalization. Medecine Science.
- Buchon, N., Broderick, N. A., & Lemaitre, B. (2013). Gut homeostasis in a microbial world: insights from Drosophila melanogaster. Nature Reviews Microbiology. 11:615-626.